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2022 ESID Medium-Term Fellowship April report

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By Victor Sosa

During my fellowship in the Center for Translational Cell Research (ZTZ) – Center for Chronic Immunodeficiency, I was working under the supervision of Prof. Dr. med. Klaus Warnatz and Dr. Bärbel Keller. I developed a project to characterize the origin and role of dysregulated tumor necrosis factor receptors (TNFR) expression in the CD21Low B-cell subpopulation, which is expanded in patients with common variable immunodeficiency (CVID).

After studying the literature and testing of available antibody clones for flow cytometry, I focused on three members of the TNF receptor superfamily (TNFRSF) TNF receptor 1A (TNFR1A), TNF receptor 1B (TNFR1B) and lymphotoxin β receptor (LTβR).  We recruited 14 CVID patients and 10 healthy individuals as controls. I established a 10-color flow cytometric staining panel to determine the expression density in different compartments of B cells from peripheral blood samples. Corroborating RNAseq data, TNFR1B and LTβ receptor protein expression were found to be increased on CD21Low B cells. TNFR1A was found without a noticeable increase between the CD21Low B cells and the other B-cell subsets.

The full report is available here